DecodeME_brain_tsv_supercluster Gene-Tissue Expression and Enrichment Report

Run Configuration

RUN_NAMEDecodeME_brain_tsv_supercluster
K_VALUES3 8 11
STAT_METHODmedian
Z_FLAG--z-score
SORT_FLAGNone

Report Thresholds

FDR Cutoff0.05
Enrichr Combined Score Min10
Max Terms per Category5
Max Tissues Shown5
Max Genes Shown20

Table of Contents

Global Files: Expression Matrix Clustering Metrics STRING Terms Enrichr Terms

Clustering with k = 3

Resolution Visualizations (k=3)

Global Heatmap Silhouette Analysis Plot Cluster Assignments (CSV) Gene Silhouette Scores (CSV)

Cluster 1 (k=3)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 29 genes.
Average Silhouette Score: 0.112.
Highest expression is observed in: oligodendrocyte, choroid plexus epithelial cell, mammillary body, leukocyte, pericyte.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- PMID: (2010) Identification of two novel chromosome regions associated with isolated growth hormone deficiency. (PMID:21284329) (FDR: 1.93e-05); (2020) GAS5 protects against osteoporosis by targeting UPF1SMAD7 axis in osteoblast differentiation. (PMID:33006314) (FDR: 0.028); (1997) A 1.1-Mb transcript map of the hereditary hemochromatosis locus. (PMID:9149941) (FDR: 0.028) *(Total significant: 3)*
- HPO: Wellbeing measurement (EFO:0007869) (FDR: 1.86e-07); Intelligence (EFO:0004337) (FDR: 3.70e-04); Mental or behavioural disorder biomarker (EFO:0006848) (FDR: 6.20e-04); Feeling miserable measurement (EFO:0009598) (FDR: 6.50e-04); Longevity (EFO:0004300) (FDR: 0.006) ... *(Total significant: 10. See string_terms.csv for full list.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Cellular_Component_2025: Cytoplasmic Stress Granule (GO:0010494) (Adj. P: 0.012, Combined Score: 236.00) *(Total significant: 1)*
- Reactome_2022: Butyrophilin (BTN) Family Interactions R-HSA-8851680 (Adj. P: 0.010, Combined Score: 1319.91) *(Total significant: 1)*

Top Genes (by silhouette):

TRIM38 (sil: 0.30), ZNF644 (sil: 0.24), ZNFX1 (sil: 0.22), ABT1 (sil: 0.22), BTN2A2 (sil: 0.21), STAU1 (sil: 0.20), DNAJC1 (sil: 0.19), VRK2 (sil: 0.19), RC3H1 (sil: 0.17), ZNF322 (sil: 0.16), KLHL20 (sil: 0.16), FBXL4 (sil: 0.14), HFE (sil: 0.14), MMS22L (sil: 0.14), ECI2 (sil: 0.14), HMGN4 (sil: 0.13), BTN3A3 (sil: 0.11), CSE1L (sil: 0.11), ZBTB37 (sil: 0.10), H4C8 (sil: 0.08) ...

Cluster 2 (k=3)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 15 genes.
Average Silhouette Score: 0.271.
Highest expression is observed in: hippocampal dentate gyrus, medium spiny neuron, hippocampal ca4, amygdala excitatory, eccentric medium spiny neuron.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of Collateral Sprouting (GO:0048671) (Adj. P: 0.046, Combined Score: 1993.61); Positive Regulation of T-helper 2 Cell Differentiation (GO:0045630) (Adj. P: 0.046, Combined Score: 1993.61); Regulation of Immunoglobulin Mediated Immune Response (GO:0002889) (Adj. P: 0.046, Combined Score: 1993.61); Neurotransmitter Receptor Transport, Endosome to Plasma Membrane (GO:0099639) (Adj. P: 0.046, Combined Score: 1993.61); CD4-positive, Alpha-Beta T Cell Activation (GO:0035710) (Adj. P: 0.046, Combined Score: 1542.87) ... *(Total significant: 38. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Dehydroascorbic Acid Transmembrane Transporter Activity (GO:0033300) (Adj. P: 0.030, Combined Score: 1249.08); High Voltage-Gated Calcium Channel Activity (GO:0008331) (Adj. P: 0.030, Combined Score: 551.00); Ionotropic Glutamate Receptor Binding (GO:0035255) (Adj. P: 0.030, Combined Score: 500.50); Phosphatidylethanolamine Binding (GO:0008429) (Adj. P: 0.030, Combined Score: 500.50); Syntaxin-1 Binding (GO:0017075) (Adj. P: 0.030, Combined Score: 457.73) ... *(Total significant: 15. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Asymmetric, Glutamatergic, Excitatory Synapse (GO:0098985) (Adj. P: 0.033, Combined Score: 1993.61); Dendritic Spine Membrane (GO:0032591) (Adj. P: 0.033, Combined Score: 1249.08); Primary Lysosome (GO:0005766) (Adj. P: 0.035, Combined Score: 685.01); Specific Granule Lumen (GO:0035580) (Adj. P: 0.028, Combined Score: 362.32); Asymmetric Synapse (GO:0032279) (Adj. P: 0.033, Combined Score: 129.44) ... *(Total significant: 7. See enrichr_terms.csv for full list.)*
- MSigDB_Hallmark_2020: Spermatogenesis (Adj. P: 0.013, Combined Score: 124.17) *(Total significant: 1)*
- Human_Phenotype_Ontology: Personality changes (HP:0000751) (Adj. P: 0.046, Combined Score: 389.38); Leiomyosarcoma (HP:0100243) (Adj. P: 0.046, Combined Score: 315.73); Uterine leiomyosarcoma (HP:0002891) (Adj. P: 0.046, Combined Score: 315.73); Transitional cell carcinoma of the bladder (HP:0006740) (Adj. P: 0.046, Combined Score: 279.21); Hereditary nonpolyposis colorectal carcinoma (HP:0006716) (Adj. P: 0.046, Combined Score: 263.64) ... *(Total significant: 14. See enrichr_terms.csv for full list.)*
- OMIM_Disease: myocardial infarction (Adj. P: 0.024, Combined Score: 421.09); colorectal cancer (Adj. P: 0.030, Combined Score: 128.60) *(Total significant: 2)*

Top Genes (by silhouette):

CCPG1 (sil: 0.38), CACNA1E (sil: 0.36), SLC2A14 (sil: 0.36), LRRC7 (sil: 0.36), CCDC92 (sil: 0.35), NEGR1 (sil: 0.32), UNC13C (sil: 0.29), DCC (sil: 0.28), HTT (sil: 0.26), OLFM4 (sil: 0.25), CDK5RAP1 (sil: 0.23), CA10 (sil: 0.22), TNFSF4 (sil: 0.19), SHISA6 (sil: 0.15), PEBP1 (sil: 0.05) ...

Cluster 3 (k=3)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 15 genes.
Average Silhouette Score: 0.058.
Highest expression is observed in: committed oligodendrocyte precursor, oligodendrocyte precursor cell, ependymal cell, mge interneuron, astrocyte.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Molecular_Function_2025: Potassium:Proton Antiporter Activity (GO:0015386) (Adj. P: 0.039, Combined Score: 685.01); G Protein-Coupled Photoreceptor Activity (GO:0008020) (Adj. P: 0.039, Combined Score: 611.46); Sodium:Proton Antiporter Activity (GO:0015385) (Adj. P: 0.039, Combined Score: 551.00); Solute:Potassium Antiporter Activity (GO:0022821) (Adj. P: 0.039, Combined Score: 457.73); Histone Deacetylase Activity (GO:0004407) (Adj. P: 0.042, Combined Score: 315.73) ... *(Total significant: 9. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Deep venous thrombosis (HP:0002625) (Adj. P: 0.017, Combined Score: 892.01); Reduced antithrombin III activity (HP:0001976) (Adj. P: 0.017, Combined Score: 892.01) *(Total significant: 2)*

Top Genes (by silhouette):

POU3F2 (sil: 0.22), VSIG10 (sil: 0.15), ZNF664 (sil: 0.14), ZNF311 (sil: 0.14), ANKRD45 (sil: 0.13), LRRIQ3 (sil: 0.11), SUDS3 (sil: 0.11), SOX6 (sil: 0.08), SLC9C2 (sil: 0.07), GPR52 (sil: 0.00), MRPL39 (sil: -0.01), SERPINC1 (sil: -0.04), DNAH10 (sil: -0.04), TAOK3 (sil: -0.07), PCDH17 (sil: -0.12) ...

Clustering with k = 8

Resolution Visualizations (k=8)

Global Heatmap Silhouette Analysis Plot Cluster Assignments (CSV) Gene Silhouette Scores (CSV)

Cluster 1 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 12 genes.
Average Silhouette Score: 0.207.
Highest expression is observed in: oligodendrocyte, leukocyte, endothelial cell, central nervous system macrophage, fibroblast.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- HPO: Wellbeing measurement (EFO:0007869) (FDR: 1.54e-05) *(Total significant: 1)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Intracellular mRNA Localization (GO:0008298) (Adj. P: 0.045, Combined Score: 2638.91); Regulation of Interleukin-1-Mediated Signaling Pathway (GO:2000659) (Adj. P: 0.045, Combined Score: 2044.88); Cellular Response to Iron Ion (GO:0071281) (Adj. P: 0.045, Combined Score: 2044.88); Negative Regulation of CD8-positive, Alpha-Beta T Cell Activation (GO:2001186) (Adj. P: 0.045, Combined Score: 1657.40); Anterograde Dendritic Transport (GO:0098937) (Adj. P: 0.045, Combined Score: 1657.40) ... *(Total significant: 37. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Cytoplasmic Stress Granule (GO:0010494) (Adj. P: 0.040, Combined Score: 329.90) *(Total significant: 1)*
- MSigDB_Hallmark_2020: Fatty Acid Metabolism (Adj. P: 0.027, Combined Score: 141.12) *(Total significant: 1)*
- Human_Phenotype_Ontology: Testicular atrophy (HP:0000029) (Adj. P: 0.039, Combined Score: 1385.98); Arthropathy (HP:0003040) (Adj. P: 0.039, Combined Score: 1032.95); Azoospermia (HP:0000027) (Adj. P: 0.039, Combined Score: 735.02); Abnormal glucose tolerance (HP:0001952) (Adj. P: 0.039, Combined Score: 668.13); Cerebral palsy (HP:0100021) (Adj. P: 0.039, Combined Score: 668.13) ... *(Total significant: 17. See enrichr_terms.csv for full list.)*
- OMIM_Disease: alzheimer disease (Adj. P: 0.031, Combined Score: 302.52); diabetes (Adj. P: 0.044, Combined Score: 76.36) *(Total significant: 2)*

Top Genes (by silhouette):

ZNFX1 (sil: 0.28), ZNF644 (sil: 0.27), DNAJC1 (sil: 0.27), MMS22L (sil: 0.24), TRIM38 (sil: 0.22), HFE (sil: 0.21), VRK2 (sil: 0.21), STAU1 (sil: 0.20), ECI2 (sil: 0.17), ZBTB37 (sil: 0.17), ABT1 (sil: 0.14), PRDX6 (sil: 0.12) ...

Cluster 2 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 6 genes.
Average Silhouette Score: 0.024.
Highest expression is observed in: endothelial cell, leukocyte, fibroblast, mammillary body, deep-layer intratelencephalic.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- PMID: (2010) Identification of two novel chromosome regions associated with isolated growth hormone deficiency. (PMID:21284329) (FDR: 4.05e-06) *(Total significant: 1)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Positive Regulation of Peroxisome Proliferator Activated Receptor Signaling Pathway (GO:0035360) (Adj. P: 0.024, Combined Score: 6499.56); Negative Regulation of Nitric Oxide Biosynthetic Process (GO:0045019) (Adj. P: 0.024, Combined Score: 3445.29); Negative Regulation of Nitric Oxide Metabolic Process (GO:1904406) (Adj. P: 0.024, Combined Score: 3445.29); NAD Biosynthesis via Nicotinamide Riboside Salvage Pathway (GO:0034356) (Adj. P: 0.024, Combined Score: 2955.69); Central Nervous System Neuron Axonogenesis (GO:0021955) (Adj. P: 0.024, Combined Score: 2955.69) ... *(Total significant: 65. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: GABA Receptor Binding (GO:0050811) (Adj. P: 0.010, Combined Score: 2580.41); Galactosyltransferase Activity (GO:0008378) (Adj. P: 0.010, Combined Score: 2580.41); UDP-galactosyltransferase Activity (GO:0035250) (Adj. P: 0.012, Combined Score: 1012.63); Protein Kinase A Regulatory Subunit Binding (GO:0034237) (Adj. P: 0.012, Combined Score: 904.00); Hexosyltransferase Activity (GO:0016758) (Adj. P: 0.041, Combined Score: 143.29) *(Total significant: 5)*
- Reactome_2022: Eicosanoids R-HSA-211979 (Adj. P: 0.028, Combined Score: 2044.88); Butyrophilin (BTN) Family Interactions R-HSA-8851680 (Adj. P: 0.028, Combined Score: 2044.88); Synthesis Of Bile Acids And Bile Salts Via 24-Hydroxycholesterol R-HSA-193775 (Adj. P: 0.028, Combined Score: 1682.80); N-Glycan Antennae Elongation R-HSA-975577 (Adj. P: 0.028, Combined Score: 1542.87); Synthesis Of Prostaglandins (PG) And Thromboxanes (TX) R-HSA-2162123 (Adj. P: 0.028, Combined Score: 1542.87) ... *(Total significant: 19. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Tetraparesis (HP:0002273) (Adj. P: 0.013, Combined Score: 2955.69); Heterotopia (HP:0002282) (Adj. P: 0.013, Combined Score: 1318.68); Poor eye contact (HP:0000817) (Adj. P: 0.013, Combined Score: 1227.64); Abnormal social behavior (HP:0012433) (Adj. P: 0.013, Combined Score: 904.00); Impaired social interactions (HP:0000735) (Adj. P: 0.013, Combined Score: 904.00) ... *(Total significant: 10. See enrichr_terms.csv for full list.)*
- OMIM_Disease: microcephaly (Adj. P: 0.008, Combined Score: 1682.80); hypertension (Adj. P: 0.009, Combined Score: 677.12) *(Total significant: 2)*

Top Genes (by silhouette):

DDX27 (sil: 0.09), BTN3A3 (sil: 0.04), ARFGEF2 (sil: 0.03), PTGIS (sil: 0.00), B4GALT5 (sil: -0.01), CSE1L (sil: -0.02) ...

Cluster 3 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 3 genes.
Average Silhouette Score: 0.095.
Highest expression is observed in: oligodendrocyte, mammillary body, pericyte, midbrain-derived inhibitory, choroid plexus epithelial cell.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Regulation of Synaptic Transmission, GABAergic (GO:0032228) (Adj. P: 0.015, Combined Score: 3054.59); Gamma-Aminobutyric Acid Signaling Pathway (GO:0007214) (Adj. P: 0.015, Combined Score: 2574.28); Phosphatidylinositol-Mediated Signaling (GO:0048015) (Adj. P: 0.015, Combined Score: 2574.28); Negative Regulation of Sequestering of Calcium Ion (GO:0051283) (Adj. P: 0.015, Combined Score: 1309.54); Release of Sequestered Calcium Ion Into Cytosol (GO:0051209) (Adj. P: 0.015, Combined Score: 1198.68) ... *(Total significant: 14. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Phosphatidylinositol Phospholipase C Activity (GO:0004435) (Adj. P: 0.008, Combined Score: 2443.77); Phospholipase C Activity (GO:0004629) (Adj. P: 0.008, Combined Score: 2216.11); Phosphoric Diester Hydrolase Activity (GO:0008081) (Adj. P: 0.009, Combined Score: 1165.42); Phospholipase Activity (GO:0004620) (Adj. P: 0.012, Combined Score: 533.11) *(Total significant: 4)*
- MSigDB_Hallmark_2020: UV Response Up (Adj. P: 0.024, Combined Score: 236.95) *(Total significant: 1)*

Top Genes (by silhouette):

HMGN4 (sil: 0.17), PLCL1 (sil: 0.12), H4C8 (sil: 0.00) ...

Cluster 4 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 8 genes.
Average Silhouette Score: 0.024.
Highest expression is observed in: choroid plexus epithelial cell, upper rhombic lip, leukocyte, mammillary body, medium spiny neuron.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- HPO: Feeling miserable measurement (EFO:0009598) (FDR: 0.004) *(Total significant: 1)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of T-helper 17 Type Immune Response (GO:2000317) (Adj. P: 0.016, Combined Score: 4436.86); - Reg of Adaptive Imm Resp Based on Som Recomb of Imm Rcptrs Built Frm IgSF Domains (GO:0002823) (Adj. P: 0.016, Combined Score: 3445.29); Regulation of T-helper Cell Differentiation (GO:0045622) (Adj. P: 0.016, Combined Score: 3445.29); tRNA Aminoacylation for Mitochondrial Protein Translation (GO:0070127) (Adj. P: 0.016, Combined Score: 2797.66); Regulation of Antigen Receptor-Mediated Signaling Pathway (GO:0050854) (Adj. P: 0.016, Combined Score: 2797.66) ... *(Total significant: 50. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Ubiquitin-Like Ligase-Substrate Adaptor Activity (GO:1990756) (Adj. P: 0.023, Combined Score: 397.81) *(Total significant: 1)*
- Reactome_2022: Butyrophilin (BTN) Family Interactions R-HSA-8851680 (Adj. P: 0.017, Combined Score: 1385.98); Mitochondrial tRNA Aminoacylation R-HSA-379726 (Adj. P: 0.017, Combined Score: 682.33); tRNA Aminoacylation R-HSA-379724 (Adj. P: 0.026, Combined Score: 284.57); Neddylation R-HSA-8951664 (Adj. P: 0.017, Combined Score: 156.65); Antigen Processing: Ubiquitination And Proteasome Degradation R-HSA-983168 (Adj. P: 0.017, Combined Score: 109.42) ... *(Total significant: 9. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Abnormality of alanine metabolism (HP:0010916) (Adj. P: 0.036, Combined Score: 2343.48); Abnormality of pyruvate family amino acid metabolism (HP:0010915) (Adj. P: 0.036, Combined Score: 2343.48); Hyperalaninemia (HP:0003348) (Adj. P: 0.036, Combined Score: 2343.48); Renal tubular acidosis (HP:0001947) (Adj. P: 0.044, Combined Score: 828.65); Acute myeloid leukemia (HP:0004808) (Adj. P: 0.044, Combined Score: 608.49) ... *(Total significant: 20. See enrichr_terms.csv for full list.)*

Top Genes (by silhouette):

DARS2 (sil: 0.13), FBXL4 (sil: 0.06), KLHL20 (sil: 0.06), MLLT10 (sil: 0.03), RABGAP1L (sil: 0.02), RC3H1 (sil: 0.01), ZNF322 (sil: -0.05), BTN2A2 (sil: -0.08) ...

Cluster 5 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 15 genes.
Average Silhouette Score: 0.221.
Highest expression is observed in: hippocampal dentate gyrus, medium spiny neuron, hippocampal ca4, amygdala excitatory, eccentric medium spiny neuron.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of Collateral Sprouting (GO:0048671) (Adj. P: 0.046, Combined Score: 1993.61); Positive Regulation of T-helper 2 Cell Differentiation (GO:0045630) (Adj. P: 0.046, Combined Score: 1993.61); Regulation of Immunoglobulin Mediated Immune Response (GO:0002889) (Adj. P: 0.046, Combined Score: 1993.61); Neurotransmitter Receptor Transport, Endosome to Plasma Membrane (GO:0099639) (Adj. P: 0.046, Combined Score: 1993.61); CD4-positive, Alpha-Beta T Cell Activation (GO:0035710) (Adj. P: 0.046, Combined Score: 1542.87) ... *(Total significant: 38. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Dehydroascorbic Acid Transmembrane Transporter Activity (GO:0033300) (Adj. P: 0.030, Combined Score: 1249.08); High Voltage-Gated Calcium Channel Activity (GO:0008331) (Adj. P: 0.030, Combined Score: 551.00); Ionotropic Glutamate Receptor Binding (GO:0035255) (Adj. P: 0.030, Combined Score: 500.50); Phosphatidylethanolamine Binding (GO:0008429) (Adj. P: 0.030, Combined Score: 500.50); Syntaxin-1 Binding (GO:0017075) (Adj. P: 0.030, Combined Score: 457.73) ... *(Total significant: 15. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Asymmetric, Glutamatergic, Excitatory Synapse (GO:0098985) (Adj. P: 0.033, Combined Score: 1993.61); Dendritic Spine Membrane (GO:0032591) (Adj. P: 0.033, Combined Score: 1249.08); Primary Lysosome (GO:0005766) (Adj. P: 0.035, Combined Score: 685.01); Specific Granule Lumen (GO:0035580) (Adj. P: 0.028, Combined Score: 362.32); Asymmetric Synapse (GO:0032279) (Adj. P: 0.033, Combined Score: 129.44) ... *(Total significant: 7. See enrichr_terms.csv for full list.)*
- MSigDB_Hallmark_2020: Spermatogenesis (Adj. P: 0.013, Combined Score: 124.17) *(Total significant: 1)*
- Human_Phenotype_Ontology: Personality changes (HP:0000751) (Adj. P: 0.046, Combined Score: 389.38); Leiomyosarcoma (HP:0100243) (Adj. P: 0.046, Combined Score: 315.73); Uterine leiomyosarcoma (HP:0002891) (Adj. P: 0.046, Combined Score: 315.73); Transitional cell carcinoma of the bladder (HP:0006740) (Adj. P: 0.046, Combined Score: 279.21); Hereditary nonpolyposis colorectal carcinoma (HP:0006716) (Adj. P: 0.046, Combined Score: 263.64) ... *(Total significant: 14. See enrichr_terms.csv for full list.)*
- OMIM_Disease: myocardial infarction (Adj. P: 0.024, Combined Score: 421.09); colorectal cancer (Adj. P: 0.030, Combined Score: 128.60) *(Total significant: 2)*

Top Genes (by silhouette):

CACNA1E (sil: 0.34), LRRC7 (sil: 0.32), CCPG1 (sil: 0.28), NEGR1 (sil: 0.27), CCDC92 (sil: 0.27), SLC2A14 (sil: 0.27), UNC13C (sil: 0.26), DCC (sil: 0.25), OLFM4 (sil: 0.24), CA10 (sil: 0.20), CDK5RAP1 (sil: 0.18), HTT (sil: 0.17), TNFSF4 (sil: 0.12), SHISA6 (sil: 0.10), PEBP1 (sil: 0.03) ...

Cluster 6 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 4 genes.
Average Silhouette Score: 0.312.
Highest expression is observed in: ependymal cell, choroid plexus epithelial cell, medium spiny neuron, hippocampal dentate gyrus, astrocyte.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- NetworkNeighborAL: Mixed, incl. Serine incorporator (Serinc), and Na,K-Atpase Interacting protein (CL:31379) (FDR: 0.006) *(Total significant: 1)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Cilium Movement Involved in Cell Motility (GO:0060294) (Adj. P: 0.018, Combined Score: 1935.63); Sodium Ion Import Across Plasma Membrane (GO:0098719) (Adj. P: 0.018, Combined Score: 1629.20); Cilium-Dependent Cell Motility (GO:0060285) (Adj. P: 0.018, Combined Score: 1337.30); Cilium Movement (GO:0003341) (Adj. P: 0.031, Combined Score: 469.28); Sodium Ion Transmembrane Transport (GO:0035725) (Adj. P: 0.032, Combined Score: 313.14) ... *(Total significant: 8. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Potassium:Proton Antiporter Activity (GO:0015386) (Adj. P: 0.005, Combined Score: 4077.19); Sodium:Proton Antiporter Activity (GO:0015385) (Adj. P: 0.005, Combined Score: 3304.67); Solute:Potassium Antiporter Activity (GO:0022821) (Adj. P: 0.005, Combined Score: 2764.28); Metal Cation:Proton Antiporter Activity (GO:0051139) (Adj. P: 0.005, Combined Score: 1822.54); Sodium Ion Transmembrane Transporter Activity (GO:0015081) (Adj. P: 0.008, Combined Score: 824.08) *(Total significant: 5)*
- GO_Cellular_Component_2025: Motile Cilium (GO:0031514) (Adj. P: 0.028, Combined Score: 322.45); 9+2 Motile Cilium (GO:0097729) (Adj. P: 0.028, Combined Score: 170.76) *(Total significant: 2)*

Top Genes (by silhouette):

ANKRD45 (sil: 0.34), LRRIQ3 (sil: 0.31), DNAH10 (sil: 0.30), SLC9C2 (sil: 0.30) ...

Cluster 7 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 5 genes.
Average Silhouette Score: 0.086.
Highest expression is observed in: committed oligodendrocyte precursor, oligodendrocyte precursor cell, ependymal cell, mge interneuron, bergmann glia.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of Cardiac Muscle Cell Differentiation (GO:2000726) (Adj. P: 0.017, Combined Score: 8352.53); Negative Regulation of Cardiocyte Differentiation (GO:1905208) (Adj. P: 0.017, Combined Score: 6499.56); Neuron Fate Specification (GO:0048665) (Adj. P: 0.017, Combined Score: 4436.86); Regulation of Cardiac Muscle Cell Differentiation (GO:2000725) (Adj. P: 0.017, Combined Score: 3808.55); Cell Differentiation in Spinal Cord (GO:0021515) (Adj. P: 0.017, Combined Score: 3808.55) ... *(Total significant: 33. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Histone Deacetylase Activity (GO:0004407) (Adj. P: 0.050, Combined Score: 1393.12); Histone Deacetylase Binding (GO:0042826) (Adj. P: 0.050, Combined Score: 185.84); Sequence-Specific Double-Stranded DNA Binding (GO:1990837) (Adj. P: 0.050, Combined Score: 81.43); Cis-Regulatory Region Sequence-Specific DNA Binding (GO:0000987) (Adj. P: 0.050, Combined Score: 45.59); RNA Polymerase II Cis-Regulatory Region Sequence-Specific DNA Binding (GO:0000978) (Adj. P: 0.050, Combined Score: 44.31) *(Total significant: 5)*

Top Genes (by silhouette):

SUDS3 (sil: 0.16), VSIG10 (sil: 0.12), POU3F2 (sil: 0.12), SOX6 (sil: 0.10), PCDH17 (sil: -0.06) ...

Cluster 8 (k=8)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 6 genes.
Average Silhouette Score: 0.006.
Highest expression is observed in: oligodendrocyte precursor cell, central nervous system macrophage, leukocyte, astrocyte, oligodendrocyte.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Molecular_Function_2025: G Protein-Coupled Photoreceptor Activity (GO:0008020) (Adj. P: 0.043, Combined Score: 2044.88); Kinase Inhibitor Activity (GO:0019210) (Adj. P: 0.048, Combined Score: 483.15); Protein Kinase Inhibitor Activity (GO:0004860) (Adj. P: 0.048, Combined Score: 283.90); Serine-Type Endopeptidase Inhibitor Activity (GO:0004867) (Adj. P: 0.048, Combined Score: 271.98); Protein Kinase Regulator Activity (GO:0019887) (Adj. P: 0.048, Combined Score: 120.65) ... *(Total significant: 9. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Deep venous thrombosis (HP:0002625) (Adj. P: 0.007, Combined Score: 2955.69); Reduced antithrombin III activity (HP:0001976) (Adj. P: 0.007, Combined Score: 2955.69); Pulmonary embolism (HP:0002204) (Adj. P: 0.022, Combined Score: 402.08) *(Total significant: 3)*

Top Genes (by silhouette):

MRPL39 (sil: 0.08), ZNF311 (sil: 0.03), SERPINC1 (sil: 0.01), GPR52 (sil: 0.00), ZNF664 (sil: -0.03), TAOK3 (sil: -0.05) ...

Clustering with k = 11

Resolution Visualizations (k=11)

Global Heatmap Silhouette Analysis Plot Cluster Assignments (CSV) Gene Silhouette Scores (CSV)

Cluster 1 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 12 genes.
Average Silhouette Score: 0.123.
Highest expression is observed in: oligodendrocyte, leukocyte, endothelial cell, central nervous system macrophage, fibroblast.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- HPO: Wellbeing measurement (EFO:0007869) (FDR: 1.54e-05) *(Total significant: 1)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Intracellular mRNA Localization (GO:0008298) (Adj. P: 0.045, Combined Score: 2638.91); Regulation of Interleukin-1-Mediated Signaling Pathway (GO:2000659) (Adj. P: 0.045, Combined Score: 2044.88); Cellular Response to Iron Ion (GO:0071281) (Adj. P: 0.045, Combined Score: 2044.88); Negative Regulation of CD8-positive, Alpha-Beta T Cell Activation (GO:2001186) (Adj. P: 0.045, Combined Score: 1657.40); Anterograde Dendritic Transport (GO:0098937) (Adj. P: 0.045, Combined Score: 1657.40) ... *(Total significant: 37. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Cytoplasmic Stress Granule (GO:0010494) (Adj. P: 0.040, Combined Score: 329.90) *(Total significant: 1)*
- MSigDB_Hallmark_2020: Fatty Acid Metabolism (Adj. P: 0.027, Combined Score: 141.12) *(Total significant: 1)*
- Human_Phenotype_Ontology: Testicular atrophy (HP:0000029) (Adj. P: 0.039, Combined Score: 1385.98); Arthropathy (HP:0003040) (Adj. P: 0.039, Combined Score: 1032.95); Azoospermia (HP:0000027) (Adj. P: 0.039, Combined Score: 735.02); Abnormal glucose tolerance (HP:0001952) (Adj. P: 0.039, Combined Score: 668.13); Cerebral palsy (HP:0100021) (Adj. P: 0.039, Combined Score: 668.13) ... *(Total significant: 17. See enrichr_terms.csv for full list.)*
- OMIM_Disease: alzheimer disease (Adj. P: 0.031, Combined Score: 302.52); diabetes (Adj. P: 0.044, Combined Score: 76.36) *(Total significant: 2)*

Top Genes (by silhouette):

DNAJC1 (sil: 0.23), ZNFX1 (sil: 0.21), ZBTB37 (sil: 0.16), ZNF644 (sil: 0.13), VRK2 (sil: 0.13), PRDX6 (sil: 0.12), HFE (sil: 0.12), TRIM38 (sil: 0.12), STAU1 (sil: 0.11), MMS22L (sil: 0.08), ECI2 (sil: 0.04), ABT1 (sil: 0.02) ...

Cluster 2 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 2 genes.
Average Silhouette Score: 0.039.
Highest expression is observed in: endothelial cell, fibroblast, mammillary body, choroid plexus epithelial cell, deep-layer intratelencephalic.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Top Genes (by silhouette):

PTGIS (sil: 0.06), ARFGEF2 (sil: 0.02) ...

Cluster 3 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 4 genes.
Average Silhouette Score: 0.054.
Highest expression is observed in: leukocyte, cerebellar inhibitory, endothelial cell, upper rhombic lip, astrocyte.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Central Nervous System Neuron Axonogenesis (GO:0021955) (Adj. P: 0.022, Combined Score: 5264.00); Central Nervous System Myelination (GO:0022010) (Adj. P: 0.022, Combined Score: 4077.19); Ganglioside Biosynthetic Process (GO:0001574) (Adj. P: 0.022, Combined Score: 2764.28); Central Nervous System Neuron Development (GO:0021954) (Adj. P: 0.022, Combined Score: 2764.28); Lymphocyte Mediated Immunity (GO:0002449) (Adj. P: 0.022, Combined Score: 2366.46) ... *(Total significant: 33. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Galactosyltransferase Activity (GO:0008378) (Adj. P: 0.006, Combined Score: 4600.93); UDP-galactosyltransferase Activity (GO:0035250) (Adj. P: 0.006, Combined Score: 1822.54); Hexosyltransferase Activity (GO:0016758) (Adj. P: 0.020, Combined Score: 265.95) *(Total significant: 3)*
- Reactome_2022: Butyrophilin (BTN) Family Interactions R-HSA-8851680 (Adj. P: 0.021, Combined Score: 3653.70); N-Glycan Antennae Elongation R-HSA-975577 (Adj. P: 0.021, Combined Score: 2764.28); N-glycan Antennae Elongation In medial/trans-Golgi R-HSA-975576 (Adj. P: 0.021, Combined Score: 1400.90); Keratan Sulfate Biosynthesis R-HSA-2022854 (Adj. P: 0.021, Combined Score: 1278.76); Keratan Sulfate/Keratin Metabolism R-HSA-1638074 (Adj. P: 0.021, Combined Score: 1044.60) ... *(Total significant: 8. See enrichr_terms.csv for full list.)*
- MSigDB_Hallmark_2020: TNF-alpha Signaling via NF-kB (Adj. P: 0.039, Combined Score: 107.24); E2F Targets (Adj. P: 0.039, Combined Score: 107.24) *(Total significant: 2)*

Top Genes (by silhouette):

DDX27 (sil: 0.15), BTN3A3 (sil: 0.03), CSE1L (sil: 0.02), B4GALT5 (sil: 0.02) ...

Cluster 4 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 3 genes.
Average Silhouette Score: 0.078.
Highest expression is observed in: oligodendrocyte, mammillary body, pericyte, midbrain-derived inhibitory, choroid plexus epithelial cell.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Regulation of Synaptic Transmission, GABAergic (GO:0032228) (Adj. P: 0.015, Combined Score: 3054.59); Gamma-Aminobutyric Acid Signaling Pathway (GO:0007214) (Adj. P: 0.015, Combined Score: 2574.28); Phosphatidylinositol-Mediated Signaling (GO:0048015) (Adj. P: 0.015, Combined Score: 2574.28); Negative Regulation of Sequestering of Calcium Ion (GO:0051283) (Adj. P: 0.015, Combined Score: 1309.54); Release of Sequestered Calcium Ion Into Cytosol (GO:0051209) (Adj. P: 0.015, Combined Score: 1198.68) ... *(Total significant: 14. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Phosphatidylinositol Phospholipase C Activity (GO:0004435) (Adj. P: 0.008, Combined Score: 2443.77); Phospholipase C Activity (GO:0004629) (Adj. P: 0.008, Combined Score: 2216.11); Phosphoric Diester Hydrolase Activity (GO:0008081) (Adj. P: 0.009, Combined Score: 1165.42); Phospholipase Activity (GO:0004620) (Adj. P: 0.012, Combined Score: 533.11) *(Total significant: 4)*
- MSigDB_Hallmark_2020: UV Response Up (Adj. P: 0.024, Combined Score: 236.95) *(Total significant: 1)*

Top Genes (by silhouette):

HMGN4 (sil: 0.14), PLCL1 (sil: 0.10), H4C8 (sil: 0.00) ...

Cluster 5 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 4 genes.
Average Silhouette Score: 0.139.
Highest expression is observed in: central nervous system macrophage, leukocyte, endothelial cell, oligodendrocyte, hippocampal dentate gyrus.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Golgi to Endosome Transport (GO:0006895) (Adj. P: 0.028, Combined Score: 2764.28); Response to Interferon-Alpha (GO:0035455) (Adj. P: 0.028, Combined Score: 2062.22); Negative Regulation of Macroautophagy (GO:0016242) (Adj. P: 0.028, Combined Score: 1400.90); Regulation of Mitophagy (GO:1901524) (Adj. P: 0.028, Combined Score: 1278.76); Regulation of Activated T Cell Proliferation (GO:0046006) (Adj. P: 0.028, Combined Score: 1044.60) ... *(Total significant: 16. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Ubiquitin-Like Ligase-Substrate Adaptor Activity (GO:1990756) (Adj. P: 0.001, Combined Score: 1455.03) *(Total significant: 1)*
- GO_Cellular_Component_2025: cullin-RING Ubiquitin Ligase Complex (GO:0031461) (Adj. P: 0.006, Combined Score: 661.38); SCF Ubiquitin Ligase Complex (GO:0019005) (Adj. P: 0.032, Combined Score: 520.17); Cul3-RING Ubiquitin Ligase Complex (GO:0031463) (Adj. P: 0.032, Combined Score: 520.17); Mitochondrial Intermembrane Space (GO:0005758) (Adj. P: 0.032, Combined Score: 404.19) *(Total significant: 4)*
- Reactome_2022: Butyrophilin (BTN) Family Interactions R-HSA-8851680 (Adj. P: 0.004, Combined Score: 3653.70); Adaptive Immune System R-HSA-1280218 (Adj. P: 0.002, Combined Score: 678.10); Neddylation R-HSA-8951664 (Adj. P: 0.003, Combined Score: 596.95); Antigen Processing: Ubiquitination And Proteasome Degradation R-HSA-983168 (Adj. P: 0.004, Combined Score: 425.14); Class I MHC Mediated Antigen Processing And Presentation R-HSA-983169 (Adj. P: 0.004, Combined Score: 322.13) ... *(Total significant: 8. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Abnormality of alanine metabolism (HP:0010916) (Adj. P: 0.015, Combined Score: 6128.34); Abnormality of pyruvate family amino acid metabolism (HP:0010915) (Adj. P: 0.015, Combined Score: 6128.34); Hyperalaninemia (HP:0003348) (Adj. P: 0.015, Combined Score: 6128.34); Renal tubular acidosis (HP:0001947) (Adj. P: 0.020, Combined Score: 2204.79); Abnormality of serum amino acid levels (HP:0003112) (Adj. P: 0.020, Combined Score: 1278.76) ... *(Total significant: 27. See enrichr_terms.csv for full list.)*

Top Genes (by silhouette):

FBXL4 (sil: 0.22), KLHL20 (sil: 0.18), ZNF322 (sil: 0.09), BTN2A2 (sil: 0.06) ...

Cluster 6 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 4 genes.
Average Silhouette Score: 0.041.
Highest expression is observed in: choroid plexus epithelial cell, upper rhombic lip, leukocyte, mammillary body, medium spiny neuron.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of T-helper 17 Type Immune Response (GO:2000317) (Adj. P: 0.010, Combined Score: 11508.86); - Reg of Adaptive Imm Resp Based on Som Recomb of Imm Rcptrs Built Frm IgSF Domains (GO:0002823) (Adj. P: 0.010, Combined Score: 8963.74); Regulation of T-helper Cell Differentiation (GO:0045622) (Adj. P: 0.010, Combined Score: 8963.74); tRNA Aminoacylation for Mitochondrial Protein Translation (GO:0070127) (Adj. P: 0.010, Combined Score: 7298.30); Regulation of Antigen Receptor-Mediated Signaling Pathway (GO:0050854) (Adj. P: 0.010, Combined Score: 7298.30) ... *(Total significant: 44. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: RNA Stem-Loop Binding (GO:0035613) (Adj. P: 0.041, Combined Score: 3653.70); miRNA Binding (GO:0035198) (Adj. P: 0.041, Combined Score: 1174.65); aminoacyl-tRNA Ligase Activity (GO:0004812) (Adj. P: 0.041, Combined Score: 877.91); Regulatory RNA Binding (GO:0061980) (Adj. P: 0.041, Combined Score: 753.68); tRNA Binding (GO:0000049) (Adj. P: 0.041, Combined Score: 595.71) ... *(Total significant: 6. See enrichr_terms.csv for full list.)*
- Reactome_2022: Mitochondrial tRNA Aminoacylation R-HSA-379726 (Adj. P: 0.017, Combined Score: 1822.54); tRNA Aminoacylation R-HSA-379724 (Adj. P: 0.017, Combined Score: 775.91) *(Total significant: 2)*
- MSigDB_Hallmark_2020: Spermatogenesis (Adj. P: 0.039, Combined Score: 178.95); IL-2/STAT5 Signaling (Adj. P: 0.039, Combined Score: 107.95); KRAS Signaling Up (Adj. P: 0.039, Combined Score: 107.24) *(Total significant: 3)*
- Human_Phenotype_Ontology: Acute myeloid leukemia (HP:0004808) (Adj. P: 0.032, Combined Score: 1629.20); Slow progression (HP:0003677) (Adj. P: 0.050, Combined Score: 269.39); Babinski sign (HP:0003487) (Adj. P: 0.050, Combined Score: 205.96); Variable expressivity (HP:0003828) (Adj. P: 0.050, Combined Score: 164.66) *(Total significant: 4)*

Top Genes (by silhouette):

RABGAP1L (sil: 0.13), MLLT10 (sil: 0.08), RC3H1 (sil: 0.01), DARS2 (sil: -0.05) ...

Cluster 7 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 3 genes.
Average Silhouette Score: 0.077.
Highest expression is observed in: upper rhombic lip, midbrain-derived inhibitory, oligodendrocyte precursor cell, lower rhombic lip, cerebellar inhibitory.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- TISSUES: Cerebellum (BTO:0000232) (FDR: 0.045) *(Total significant: 1)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Synaptic Vesicle Docking (GO:0016081) (Adj. P: 0.013, Combined Score: 8255.55); Synaptic Transmission, Glutamatergic (GO:0035249) (Adj. P: 0.019, Combined Score: 2216.11); Synaptic Vesicle Exocytosis (GO:0016079) (Adj. P: 0.021, Combined Score: 1198.68); Negative Regulation of MAPK Cascade (GO:0043409) (Adj. P: 0.044, Combined Score: 312.81); Brain Development (GO:0007420) (Adj. P: 0.044, Combined Score: 215.73) ... *(Total significant: 10. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Phosphatidylethanolamine Binding (GO:0008429) (Adj. P: 0.007, Combined Score: 4739.66); Syntaxin-1 Binding (GO:0017075) (Adj. P: 0.007, Combined Score: 4351.69); Hydro-Lyase Activity (GO:0016836) (Adj. P: 0.010, Combined Score: 1103.68); Syntaxin Binding (GO:0019905) (Adj. P: 0.010, Combined Score: 1103.68) *(Total significant: 4)*
- GO_Cellular_Component_2025: Neuromuscular Junction (GO:0031594) (Adj. P: 0.017, Combined Score: 1394.27); Exocytic Vesicle Membrane (GO:0099501) (Adj. P: 0.017, Combined Score: 695.09); Synaptic Vesicle Membrane (GO:0030672) (Adj. P: 0.017, Combined Score: 682.49) *(Total significant: 3)*
- Reactome_2022: Signaling By High-Kinase Activity BRAF Mutants R-HSA-6802948 (Adj. P: 0.018, Combined Score: 1656.47); MAP2K And MAPK Activation R-HSA-5674135 (Adj. P: 0.018, Combined Score: 1440.46); Paradoxical Activation Of RAF Signaling By Kinase Inactive BRAF R-HSA-6802955 (Adj. P: 0.018, Combined Score: 1270.59); Negative Regulation Of MAPK Pathway R-HSA-5675221 (Adj. P: 0.018, Combined Score: 1270.59); Signaling By BRAF And RAF1 Fusions R-HSA-6802952 (Adj. P: 0.022, Combined Score: 764.89) ... *(Total significant: 6. See enrichr_terms.csv for full list.)*
- MSigDB_Hallmark_2020: Spermatogenesis (Adj. P: 0.020, Combined Score: 289.52) *(Total significant: 1)*

Top Genes (by silhouette):

PEBP1 (sil: 0.15), UNC13C (sil: 0.06), CA10 (sil: 0.02) ...

Cluster 8 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 12 genes.
Average Silhouette Score: 0.227.
Highest expression is observed in: medium spiny neuron, hippocampal dentate gyrus, hippocampal ca4, amygdala excitatory, eccentric medium spiny neuron.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- HPO: Self reported educational attainment (EFO:0004784) (FDR: 0.007); Depressive symptom measurement (EFO:0007006) (FDR: 0.036) *(Total significant: 2)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of Collateral Sprouting (GO:0048671) (Adj. P: 0.040, Combined Score: 2638.91); Positive Regulation of T-helper 2 Cell Differentiation (GO:0045630) (Adj. P: 0.040, Combined Score: 2638.91); Regulation of Immunoglobulin Mediated Immune Response (GO:0002889) (Adj. P: 0.040, Combined Score: 2638.91); Neurotransmitter Receptor Transport, Endosome to Plasma Membrane (GO:0099639) (Adj. P: 0.040, Combined Score: 2638.91); CD4-positive, Alpha-Beta T Cell Activation (GO:0035710) (Adj. P: 0.040, Combined Score: 2044.88) ... *(Total significant: 54. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Dehydroascorbic Acid Transmembrane Transporter Activity (GO:0033300) (Adj. P: 0.033, Combined Score: 1657.40); High Voltage-Gated Calcium Channel Activity (GO:0008331) (Adj. P: 0.033, Combined Score: 735.02); Ionotropic Glutamate Receptor Binding (GO:0035255) (Adj. P: 0.033, Combined Score: 668.13); Hexose Transmembrane Transporter Activity (GO:0015149) (Adj. P: 0.033, Combined Score: 562.89); Glutamate Receptor Binding (GO:0035254) (Adj. P: 0.033, Combined Score: 397.51) ... *(Total significant: 9. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Asymmetric, Glutamatergic, Excitatory Synapse (GO:0098985) (Adj. P: 0.019, Combined Score: 2638.91); Dendritic Spine Membrane (GO:0032591) (Adj. P: 0.019, Combined Score: 1657.40); Primary Lysosome (GO:0005766) (Adj. P: 0.025, Combined Score: 912.35); Specific Granule Lumen (GO:0035580) (Adj. P: 0.016, Combined Score: 502.06); AMPA Glutamate Receptor Complex (GO:0032281) (Adj. P: 0.040, Combined Score: 374.56) ... *(Total significant: 15. See enrichr_terms.csv for full list.)*
- Reactome_2022: Netrin Mediated Repulsion Signals R-HSA-418886 (Adj. P: 0.045, Combined Score: 1385.98); Regulation Of Commissural Axon Pathfinding By SLIT And ROBO R-HSA-428542 (Adj. P: 0.045, Combined Score: 1185.99); Role Of Second Messengers In Netrin-1 Signaling R-HSA-418890 (Adj. P: 0.045, Combined Score: 1032.95); Caspase Activation Via Dependence Receptors In Absence Of Ligand R-HSA-418889 (Adj. P: 0.045, Combined Score: 1032.95); DSCAM Interactions R-HSA-376172 (Adj. P: 0.045, Combined Score: 912.35) ... *(Total significant: 17. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Personality changes (HP:0000751) (Adj. P: 0.037, Combined Score: 520.84); Leiomyosarcoma (HP:0100243) (Adj. P: 0.037, Combined Score: 423.08); Uterine leiomyosarcoma (HP:0002891) (Adj. P: 0.037, Combined Score: 423.08); Transitional cell carcinoma of the bladder (HP:0006740) (Adj. P: 0.037, Combined Score: 374.56); Hereditary nonpolyposis colorectal carcinoma (HP:0006716) (Adj. P: 0.037, Combined Score: 353.87) ... *(Total significant: 17. See enrichr_terms.csv for full list.)*
- OMIM_Disease: myocardial infarction (Adj. P: 0.019, Combined Score: 562.89); colorectal cancer (Adj. P: 0.024, Combined Score: 173.93) *(Total significant: 2)*

Top Genes (by silhouette):

LRRC7 (sil: 0.33), CCPG1 (sil: 0.31), OLFM4 (sil: 0.29), DCC (sil: 0.27), CACNA1E (sil: 0.27), CCDC92 (sil: 0.25), CDK5RAP1 (sil: 0.21), HTT (sil: 0.21), NEGR1 (sil: 0.18), SLC2A14 (sil: 0.17), SHISA6 (sil: 0.12), TNFSF4 (sil: 0.10) ...

Cluster 9 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 4 genes.
Average Silhouette Score: 0.307.
Highest expression is observed in: ependymal cell, choroid plexus epithelial cell, medium spiny neuron, hippocampal dentate gyrus, astrocyte.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

STRING network enrichment:
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- NetworkNeighborAL: Mixed, incl. Serine incorporator (Serinc), and Na,K-Atpase Interacting protein (CL:31379) (FDR: 0.006) *(Total significant: 1)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Cilium Movement Involved in Cell Motility (GO:0060294) (Adj. P: 0.018, Combined Score: 1935.63); Sodium Ion Import Across Plasma Membrane (GO:0098719) (Adj. P: 0.018, Combined Score: 1629.20); Cilium-Dependent Cell Motility (GO:0060285) (Adj. P: 0.018, Combined Score: 1337.30); Cilium Movement (GO:0003341) (Adj. P: 0.031, Combined Score: 469.28); Sodium Ion Transmembrane Transport (GO:0035725) (Adj. P: 0.032, Combined Score: 313.14) ... *(Total significant: 8. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Potassium:Proton Antiporter Activity (GO:0015386) (Adj. P: 0.005, Combined Score: 4077.19); Sodium:Proton Antiporter Activity (GO:0015385) (Adj. P: 0.005, Combined Score: 3304.67); Solute:Potassium Antiporter Activity (GO:0022821) (Adj. P: 0.005, Combined Score: 2764.28); Metal Cation:Proton Antiporter Activity (GO:0051139) (Adj. P: 0.005, Combined Score: 1822.54); Sodium Ion Transmembrane Transporter Activity (GO:0015081) (Adj. P: 0.008, Combined Score: 824.08) *(Total significant: 5)*
- GO_Cellular_Component_2025: Motile Cilium (GO:0031514) (Adj. P: 0.028, Combined Score: 322.45); 9+2 Motile Cilium (GO:0097729) (Adj. P: 0.028, Combined Score: 170.76) *(Total significant: 2)*

Top Genes (by silhouette):

ANKRD45 (sil: 0.34), LRRIQ3 (sil: 0.31), SLC9C2 (sil: 0.30), DNAH10 (sil: 0.28) ...

Cluster 10 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 5 genes.
Average Silhouette Score: 0.078.
Highest expression is observed in: committed oligodendrocyte precursor, oligodendrocyte precursor cell, ependymal cell, mge interneuron, bergmann glia.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of Cardiac Muscle Cell Differentiation (GO:2000726) (Adj. P: 0.017, Combined Score: 8352.53); Negative Regulation of Cardiocyte Differentiation (GO:1905208) (Adj. P: 0.017, Combined Score: 6499.56); Neuron Fate Specification (GO:0048665) (Adj. P: 0.017, Combined Score: 4436.86); Regulation of Cardiac Muscle Cell Differentiation (GO:2000725) (Adj. P: 0.017, Combined Score: 3808.55); Cell Differentiation in Spinal Cord (GO:0021515) (Adj. P: 0.017, Combined Score: 3808.55) ... *(Total significant: 33. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Histone Deacetylase Activity (GO:0004407) (Adj. P: 0.050, Combined Score: 1393.12); Histone Deacetylase Binding (GO:0042826) (Adj. P: 0.050, Combined Score: 185.84); Sequence-Specific Double-Stranded DNA Binding (GO:1990837) (Adj. P: 0.050, Combined Score: 81.43); Cis-Regulatory Region Sequence-Specific DNA Binding (GO:0000987) (Adj. P: 0.050, Combined Score: 45.59); RNA Polymerase II Cis-Regulatory Region Sequence-Specific DNA Binding (GO:0000978) (Adj. P: 0.050, Combined Score: 44.31) *(Total significant: 5)*

Top Genes (by silhouette):

SUDS3 (sil: 0.16), VSIG10 (sil: 0.12), POU3F2 (sil: 0.12), SOX6 (sil: 0.10), PCDH17 (sil: -0.10) ...

Cluster 11 (k=11)

Gene List (TXT) Cluster Heatmap (PNG)

This cluster contains 6 genes.
Average Silhouette Score: -0.012.
Highest expression is observed in: oligodendrocyte precursor cell, central nervous system macrophage, leukocyte, astrocyte, oligodendrocyte.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

Enrichr annotation enrichment:
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Molecular_Function_2025: G Protein-Coupled Photoreceptor Activity (GO:0008020) (Adj. P: 0.043, Combined Score: 2044.88); Kinase Inhibitor Activity (GO:0019210) (Adj. P: 0.048, Combined Score: 483.15); Protein Kinase Inhibitor Activity (GO:0004860) (Adj. P: 0.048, Combined Score: 283.90); Serine-Type Endopeptidase Inhibitor Activity (GO:0004867) (Adj. P: 0.048, Combined Score: 271.98); Protein Kinase Regulator Activity (GO:0019887) (Adj. P: 0.048, Combined Score: 120.65) ... *(Total significant: 9. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Deep venous thrombosis (HP:0002625) (Adj. P: 0.007, Combined Score: 2955.69); Reduced antithrombin III activity (HP:0001976) (Adj. P: 0.007, Combined Score: 2955.69); Pulmonary embolism (HP:0002204) (Adj. P: 0.022, Combined Score: 402.08) *(Total significant: 3)*

Top Genes (by silhouette):

MRPL39 (sil: 0.04), ZNF311 (sil: 0.03), GPR52 (sil: 0.00), SERPINC1 (sil: -0.02), ZNF664 (sil: -0.03), TAOK3 (sil: -0.10) ...